Catalase Activity Catalase H,O, 0 + O2 (2) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water. An experiment investigated the effect of temperature on the rate of the catalase reaction Small (10 cm) test tubes were used for the reactions, each containing 0.5 cm of enzyme and 4 cm of hydrogen peroxide. Reaction rates were assessed at four temperatures (10°C, 20°C, 30’C. and 60 C). For each temperature, there were two reaction tubes (e.g. tubes 1 and 2 were both kept at 10°C). The height of oxygen bubbles present after one minute of 10°C 20°C 30°C 60°C Height of ????en bubbles 4 cm H,02+ 0.5 cm erzyme reaction was used as a measure of the reaction rate: a laster reaction rate produced more bubbles. The entire experiment, involving eight tubes, was repeated on two separate days. Tubes Tubes Tubes 1 & 2 Tubes 3 & 4 5 & 6 7 & 8 1. What is the purpose of this experiment? inrestrgatee effect of demperalure on the rate ot he 2. Write a hypothesis for this experiment. 3. What is the independent variable? The enzyme 2md hycrogen peroXide 4. What are the units of the independent variable? 5. What is the range of values for the independent variable? 6. What is the dependent variable? he reaedion time in He test tube 7. What is the units of the dependent variable? 8. Explain why it would have been good to include a tube that contained no enzyme. 4303 have been good because 9. List three variables that should have been controlled and how they could have been monitored.
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Catalase Activity Catalase H,O, 0 + O2 (2) Catalase is an enzyme that converts hydrogen peroxide (H,O) to oxygen and water.